In 1999, Madhuri married Sriram Madhav Nene, an Indian doctor who resides in USA and is also from a Marathi Konkanastha Brahmin family. She has two sons, Arin (born in March 2003 in Colorado) and Ryan (born on March 8, 2005 in Colorado).
She is an accomplished Kathak dancer and is trained in the dance for eight years. On February 25, 2006 Madhuri performed on stage for the first time in six years at the Filmfare Awards to music from the movie 'Devdas'.